VARIAN NON-DELESI 9 PASANG BASA DNA MITOKONDRIA MANUSIA SAMPEL FORENSIK BALI
DOI:
https://doi.org/10.18269/jpmipa.v6i1.34984Keywords:
mtDNA, 9-bp deletion, PCRAbstract
One of human mitochondrial DNA (mtDNA) variant is a 9 base pairs (bp) deletion in the COII/tRNALys intergenic region. In construction mtDNA nomenclature, 9-bp deletion database consist of primary and secondary data is needed, including Bali bombing forensic samples. Here we report a 9-bp nondeletion mtDNA variant from Bali bombing forensic samples to complete primary data. Polymerase Chain Reaction (PCR) technique with 2 set primer was used to detect 9-bp deletion. The PCR result was detected by agarose gel electrophoresis, which showed two bands (0.1 and 0.4 kb) for non-deletion variant control, and one band (0.4 kb) for deletion variant control. If the sample has 9-bp deletion, only one of the primer pairs could amplify a fragment of 0.4 kb. If the sample does not have 9-bp deletion, the other primer pair will amplify a 0.1 kb product. The result showed that none of the 24 samples has 9-bp deletion. These results are contributed to the human mtDNA database and nomenclature construction.
Downloads
References
Anderson, S., Bankier, A.T., Barrell, B.G., de Bruijn, M.H., Coulson, A.R., Drouin, J., Eperon, I.C., Nierlich, D.P., Roe, B.A, Sanger, F., Schreier, P.H., Smith, A.J., Staden, R. and Young, I.G., (1981), Sequence and organization of the human mitochondrial genome, Nature 290, 457-467.
Handoko, H.Y., Lum, J.K., Gustiani, Rismalia, Kartrapradja, H., Sofro, A.S.M. and Marzuki, S., (2001), Length Variations in COII-tRNALys Intergenic Region of Mitochondrial DNA in Indonesian Populations, Hum. Biol., 73, 205-223.
Marzuki, S., Sudoyo, H. and Noer, A.S., (1991), Polymorphism of the Human Mitochondrial DNA and Forensic medicine, Maj. Kedok. Indon., 41, 737-740.
Marzuki, S., Noer, A.S., Lertrit, P., Thyagarajan, D., Kapsa, R., Utthanaphol, P. and Byrne, E., (1991), Normal variants of human mitochondrial DNA and translation products: the building of a reference data base, Hum. Genet., 88, 139-145.
Melton T., Peterson, R., Redd, A.J., Saha, N., Sofro, A.S., Martinson, J. and Stoneking, M., (1995), Polynesian genetic affinities with Southeast Asian populations as identified by mtDNA analysis, Am. J. Hum. Genet., 57, 403-414.
Noer, A.S., Sudoyo, H., Lertrit, P., Thyagarajan, D., Utthanaphol, P., Kapsa, R., Byrne, E. and Marzuki, S., (1991), A tRNALys mutation in the mtDNA is the causal genetic lesion underlying myoclonic epilepsy and ragged-red fiber (MERRF) syndrome, Am. J. Hum. Genet., 49, 715-722.
Noer, A.S., Martasih, F., Mulyani, S., Muktiningsih dan Wirahadikusumah, M., (1994), Analisis varian urutan nukleotida D-loop mtDNA manusia dari berbagai daerah di Indonesia. Proseeding Seminar Bersama UKM-ITB, I, 201-214.
Redd A.J., Takezaki, N., Sherry S.T., McGarvey S.T., Sofro, A.S.M. and Stoneking, M., (1995) Evolutionery History of COII/tRNALys Intergenic 9 Base Pair Deletion in Human Mitochondrial DNAs from Pasific, Mol. Biol. Evol., 12, 605-615.
Stone, C., Starrs, J.E. and Stoneking, M., (2001), Mitochondrial DNA Analysis of the Presumptive Remains of Jesse James, J. Forensic Sci., 46, 173-176.
Syafrizayanti, (2004), Haplotype dan Deteksi Sederhana Delesi 9-pb Daerah Antargen COII/tRNALys DNA Mitokondria Manusia, Tesis, Bidang Studi Magister Kimia Program Pasca Sarjana ITB.
Thomas, M.G., Cook, C.E., Miller, K.W. P., Waring, M.J. and Hagelberg, E., (1998), Molecular instability in COII-tRNALys intergenic region of human mitochondrial genome: multiple origins of the 9-bp deletion and heteroplasmy for expanded repeats, Phil. Trans. R. Soc. Lond. B., 353, 955-965.
Wallace, D.C. (1997), Mitochondrial DNA in Aging and Disease. Sci. Am., August, 22-29.
Wrischnik, L.A., Higuchi, R.G., Stoneking, M., Erlichl, H.A., Arnheim, N. and Wilson A.C., (1987), Length Mutation in Human Mitochondrial DNA: Direct Sequencingof Enzimatically Amplified DNA, Nucl. Acids Res., 12, 529-542.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
